流式细胞仪检测细胞凋亡操作流程

流式检测细胞凋亡

Annexin V 检测细胞凋亡........................................................................................2 Annexin V

实验原理................................................................................................................2 实验用品................................................................................................................2 操作步骤................................................................................................................3 Annexin V Blocking..............................................................................................5 凋亡细胞的DNA断裂片段分析.............................................................................7

实验原理................................................................................................................7 实验用品................................................................................................................8 操作步骤................................................................................................................9 BrdU Flow Kits检测细胞增殖..............................................................................12 BrdU Flow

实验原理..............................................................................................................12 BrdU Flow Kits试剂盒.......................................................................................12 结果分析..............................................................................................................17 流式仪器设置指南..............................................................................................18 线粒体膜电位变化检测细胞凋亡..........................................................................22

实验原理..............................................................................................................22 实验用品..............................................................................................................22 样本制备..............................................................................................................23 结果分析..............................................................................................................24 注意事项..............................................................................................................24 Active Caspase-3检测细胞凋亡...........................................................................26 Active

实验原理..............................................................................................................26 实验步骤..............................................................................................................27 结果分析..............................................................................................................28