瓦氏黄颡鱼线粒体全基因组序列分析及系统进化

HEREDITAS (Beijing) 2011年6月, 33(6): 627―635 ISSN 0253-9772 doc.guandang.net

研究报告

DOI: 10.3724/SP.J.1005.2011.00627

李林1, 2,梁宏伟2, 3,李忠2, 3,罗相忠2, 3,呼光富1, 2,张志伟1, 2,朱媛媛1, 2, 邹桂伟1, 2, 3

1. 华中农业大学水产学院, 武汉 430070;

2. 农业部淡水生物多样性保护与利用重点开放实验室, 中国水产科学研究院长江水产研究所, 荆州 434000; 3. 中国水产科学研究院淡水渔业研究中心, 无锡 214081

摘要: 鲿科鱼类种类繁多, 外形相似, 形态学分类较为困难。为了给鲿科鱼类乃至鲇形目鱼类的系统进化研究

积累基础资料, 文章采用参照近缘物种线粒体基因组设计覆盖全基因组引物的方法, 利用16对引物对瓦氏黄颡鱼(Pelteobagrus vachelli)线粒体全基因组进行扩增, PCR产物转化到质粒后测序, 最终获得线粒体基因组全序列, 其全长为16 527 bp, 包括2个rRNA基因、22个tRNA基因、13个编码蛋白质基因和一个非编码控制区。瓦氏黄颡鱼(P. vachelli)线粒体基因组结构和基因排列顺序与现已公布的鲇形目鱼类完全一致, 序列分析表明, 与鲇形目其他种属间具有较高的同源性, 与拟鲿属的同源性最高(91%)。利用鲇形目共4科6属9种及3个外群的线粒体全基因组序列, 从线粒体基因组水平探讨了鲿科鱼类及其在鲇形目的系统进化地位, 结果表明: 鲿科鱼类的瓦氏黄颡鱼(P. vachelli)、黄颡鱼(Pelteobagrus fulvidraco)、光泽黄颡鱼(Pelteobagrus nitidus)及越南拟鲿(Pseudobagrus tokiensis)构成一单系群; 拟鲿属与黄颡鱼属的关系较近; 黄颡鱼属中瓦氏黄颡鱼(P. vachelli)与光泽黄颡鱼(P.nitidus)的关系近于黄颡鱼(P. fulvidraco)。

关键词: 瓦氏黄颡鱼; 线粒体基因组; 基因定位; 序列分析

Sequence and phylogeny analysis of the complete mitochondrial genome of Pelteobagrus vachelli

LI Lin1, 2, LIANG Hong-Wei2, 3, LI Zhong2, 3, LUO Xiang-Zhong2, 3, HU Guang-Fu1, 2, ZHANG Zhi-Wei1, 2, ZHU Yuan-Yuan1, 2, ZOU Gui-Wei1, 2, 3

1. Fisheries College of Huazhong Agricultural University, Wuhan 430070, China;

2. Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Certificated by Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Jingzhou 434000, China; 3. Freshwater fisheries Research Center of Chinese Academy of Fishery Sciences, Wuxi 214081, China

Abstract: Morphological classification of Bagridae fishes is relatively difficult due to various kinds and similar shape, and the phyletic evolution is not very clear in some species. To provide basic data to the classification of Bagridae and Siluriformes fishes, the complete mitochondrial genome of Pelteobagrus vachelli was obtained by PCR based on 16 primers, which were designed on the basis of related species mtDNA sequences. The complete mitochondrial genome is 16 527 bp in length, including 2 ribosomal RNA genes, 22 transfer RNA genes, 13 protein-coding genes, and a non-coding control region. The organization and location of genes in the mitochondrial genome of Pelteobagrus vachelli were consistent with Siluri-

收稿日期: 2010 07 27; 修回日期: 2010 10 20

基金项目:国家科技基础条件平台专项(编号：2006DKA30470—002)和中央级公益性科研院所基本科研业务费专项资助

作者简介:李林, 硕士研究生, 专业方向：鱼类遗传育种。E-mail: lilin-zyfz@163.com

通讯作者:邹桂伟, 研究员, 硕士生导师, 研究方向：鱼类遗传育种。

瓦氏黄颡鱼线粒体全基因组序列分析及系统进化