秦川牛GHR基因SNPs及其与生长性状关系的研究

利用PCR-SSCP技术研究了136头秦川牛GHR基因第10外显子多态性, 所研究的秦川牛群体GHR基因该座位存在多态性, 发现了A、B、C 3个等位基因, 其基因频率依次为0.5956、0.2905、0.1140, 并且群体处于Hardy-Weinberg平衡状态。对纯合基因型个体进行测序, 测序结果, 该座位存在两个突

HEREDITAS (Beijing)

2007年3月, 29(3): 319―323 ISSN 0253-9772 doc.guandang.net

研究报告

DOI: 10.1360/yc-007-0319

秦川牛GHR基因SNPs及其与生长性状关系的研究

赵高锋1, 陈宏1,2, 雷初朝1, 张春雷1, 张丽1, 蓝贤勇1, 房兴堂2, 康湘涛1

1. 西北农林科技大学动物科技学院, 陕西省农业分子生物学重点实验室, 杨凌 712100; 2. 徐州师范大学细胞与分子生物学研究所, 徐州 221116

摘要: 利用PCR-SSCP技术研究了136头秦川牛GHR基因第10外显子多态性, 所研究的秦川牛群体GHR基因该座位存在多态性, 发现了A、B、C 3个等位基因, 其基因频率依次为0.5956、0.2905、0.1140, 并且群体处于Hardy-Weinberg平衡状态。对纯合基因型个体进行测序, 测序结果, 该座位存在两个突变位点。通过构建最小二乘线性模型, 分析了生长激素受体基因与生长性状的关系, 表明生长激素受体基因的AB基因型效应在部分生长性状上高于其他基因型, 差异达到显著水平, 提示GHR基因有可能作为秦川牛生长性状的侯选基因。 关键词: 秦川牛; PCR-SSCP; 生长激素受体基因; 多态性

Study on SNPs of GHR gene and its associations with growth traits in Qinchuan cattle

ZHAO Gao-Feng1, CHEN Hong1,2, LEI Chu-Zhao1, ZHANG Chun-Lei1, ZHANG Li1,

LAN Xian-Yong1, FANG Xing-Tang2, KANG Xiang-Tao1

1. College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, 712100, China;

2. Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou 221116, China

Abstract: In this paper PCR-SSCP technique was used to analyze the polymorphisms of the tenth exon of GHR gene in

Qinchuan cattle. The results showed that PCR products demonstrated polymorphisms in Qinchuan cattle and the population was at Hardy-Weinberg equilibrium (P > 0.05). There were three allele gene A、B、C in this locus with the corresponding frequencies of 0.5956, 0.2905 and 0.1140. The amplified fragments of homozygosis genotype were sequenced. The result showed that there were two mutation in these fragments. Then the relationship between genotypes and productive perform-ances was analyzed by the Least Squire Method (LSM). The results showed that some productive performances of the indi-viduals with genotype AB were higher than those of the other genotypes, and the derivation was significant among geno-types. It suggested that GHR gene may be a candidate gene responsible for growth trait in Qinchuan cattle.

Keywords: Qinchuan cattle; PCR-SSCP; GHR gene; polymorphism

生长激素(Growth Hormone, GH)是调节动物生长、发育等代谢过程的重要内分泌因子。GH在组织和细胞水平发挥作用的第一步是和靶细胞膜表面的

生长激素受体(Growth Hormone Receptor, GHR) 结合, 由GHR介导将信号传入细胞内从而产生一系列的生理效应。组织中GHR 量的多少、功能的正常与

收稿日期: 2006-08-27; 修回日期: 2006-09-25

基金项目: 国家高技术研究发展计划项目（国家863计划）(编号：2006AA10Z197)和西北农林科技大学拔尖人才支持计划基金资助[Supported by Chinese National Programs for High Technology Research and Development (863 Program) (No. 2006AA10Z197) and the Outstanding Talents Foundation of Northwest A & F University]

作者简介: 赵高峰(1982—), 男, 陕西省扶风人, 硕士研究生, 专业方向: 动物遗传学。E-mail: zhaochenfeng122@163.com 通讯作者: 陈宏(1955—), 男, 教授, 博士生导师, 研究方向为生物技术与动物育种。E-mail: chenhong1212@263.net